HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NOSTRIN (Cat# RC204336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOSTRIN (Cat# TA811010)(1:2000). Positive lysates [LY403273] (100 ug) and [LC403273] (20 ug) can be purchased separately from OriGene.