HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NR1D2 (Cat# RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2(Cat# TA806588). Positive lysates [LY417483] (100 ug) and [LC417483] (20 ug) can be purchased separately from OriGene.