HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUCB1 (Cat# RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1 (Cat# TA503868). Positive lysates [LY416822] (100 ug) and [LC416822] (20 ug) can be purchased separately from OriGene.