HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUDT6 (Cat# RC203470, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUDT6(Cat# TA502343). Positive lysates [LY402086] (100 ug) and [LC402086] (20 ug) can be purchased separately from OriGene.