Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S15 antibody ( TA382720) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 180s.