WB analysis using Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. The blot was incubated with a 1:1,000 dilution of the antibody at RTfollowed by washing. A 1:20,000 dilution of HRP conj ugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molec ular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molec ular weight for human PMS2 protein.