WB using Anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. The primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.