3-mC, Rabbit Polyclonal Antibody

Partner: OriGene Technologies

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Dot blot analysis using the antibody against 3-mC To demonstrate the specificity of the antibody against 3-mC, a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo' were spotted on the membrane. The antibody was diluted :400 in PBS-T containing 10 % skimmed milk and 1% BSA. Image shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.