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Cat Number:SG03-10
Size:10 assays

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Cellular Senescence Detection Kit - SPiDER-betaGal

Partner: Dojindo EU GmbH

Description:DNA damages of the normal cells are caused by repeated cell division and oxidative stress. Cellular Senescence, a state of irreversible growth arrest, can be triggered in order to prevent DNA-damaged cells from growing. Senescence-associated beta-galactosidase (SA-beta-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Although X-gal is a well known reagent to detect SA-beta-gal, these are following disadvantages: 1) requirement of fixed cells due to the poor cell-permeability, 2) low quantitative capability because of the difficulty of the determination of visual difference between stained cells and not stained cells, 3) requirement of a long time of staining.Cellular Senescence Detection Kit � SPiDER-betaGal allows to detect SA-beta-gal with high sensitivity and ease of use. SPiDER-betaGal is a new reagent to detect beta-galactosidase which possesses a high cell-permeability and a high retentivity inside cells. SA-beta-gal are detected specifically not only in living cells but also fixed cells by using a reagent (Bafilomycin A1) to inhibit endogenous beta-galactosidase activity. Therefore, SPiDER-betaGal can be applied to quantitative analysis by flow cytometry.
Further Information:Recent work from Dr. Kim et al. at Mayo Clinic used our Cellular Senescence Detection Kit � SPiDER-betaGal to evaluate cellular senescence in endothelial cells. They did staining of SA-betaGal in cells in atherosclerotic renal artery stenosis (ARAS) and co-stained the cells with CD31 which is a marker of endothelilal cells. They showed that ARAS + Elamipretide* treatment slightly improved endothelial cell senescence. Unlike commercial available probes used for detection of beta-Galactosidase, SPiDER-betaGal contained in the kit possesses high intracellular retention. The key feature of this product is that it can be used to co-stain SA-beta-Gal and other markers. Our kit is a useful tool for cellular senescence research.
Shipping Conditions:Ship at ambient
Storage:Store at 0.5 C
Usage:Research use only


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