| Description: | DNA damages of the normal cells are caused by repeated cell division and oxidative stress. Cellular Senescence, a state of irreversible growth arrest, can be triggered in order to prevent DNA-damaged cells from growing. Senescence-associated beta-galactosidase (SA-beta-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Although X-gal is a well known reagent to detect SA-beta-gal, these are following disadvantages: 1) requirement of fixed cells due to the poor cell-permeability, 2) low quantitative capability because of the difficulty of the determination of visual difference between stained cells and not stained cells, 3) requirement of a long time of staining.Cellular Senescence Detection Kit � SPiDER-betaGal allows to detect SA-beta-gal with high sensitivity and ease of use. SPiDER-betaGal is a new reagent to detect beta-galactosidase which possesses a high cell-permeability and a high retentivity inside cells. SA-beta-gal are detected specifically not only in living cells but also fixed cells by using a reagent (Bafilomycin A1) to inhibit endogenous beta-galactosidase activity. Therefore, SPiDER-betaGal can be applied to quantitative analysis by flow cytometry. |
