Mixed neuron/glia cultures from newborn rat brain stained with peripherin (green) and phosphorylated NF-H (red). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-H, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
