Workflow of the EpiNext ChIP-Seq High-Sensitivity Kit (Illumina).
Highly sensitive ChIP: The sheared chromatin isolated from different amounts of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.
Size distribution of library fragments. Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.