Western blot analysis of extracts of MCF-7 cells, using Mast Cell Chymase antibody.
DF12290 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.