| Applications: | ELISA, WB, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2a kappa |
| Immunogen: | Recombinant human NMT1 (1-496aa) purified from E. coli. |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | The antibody has been tested by ELISA, Western blot analysis, ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
| Synonym: | Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1 |
| Ascension Number: | P30419 |
| NCBI EntrezGene: | NP_066565 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
