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Cat Number:ATGA0242-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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CISD1 [AT1A8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human CISD1 (32-108aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Synonym:CDGSH iron-sulfur domain-containing protein 1, C10orf70; MDS029; mitoNEET; ZCD1
Ascension Number:Q9NZ45
NCBI EntrezGene:NP_060934
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysate

The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysate