The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysateThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Balb-3T3 cell lysateLane 2.: HeLa cell lysateLane 3.: Raji cell lysateLane 4.: K562 cell lysateLane 5.: A549 cell lysate
ICC/IF analysis of PKLR in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKLR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of PKLR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKLR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
