All lanes : Anti-AMOTL2 Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: A375 whole cell lysateLane 3: HUVEC whole cell lysateLane 4: Caco2 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.