All lanes : Anti-ABCB5 Antibody (N-term) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: K562 whole cell lysateLane 3: HL-60 whole cell lysateLane 4: A375 whole cell lysateLane 5: A2058 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDaBlocking/Dilution buffer: 5% NFDM/TBST.