All lanes : Anti-SAPCD2 Antibody (C-Term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: PC-3 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.