All lanes : Anti-AMZ1 Antibody (N-Term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: human liver lysatesLane 3: human heart lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.