All lanes : Anti-adra2db Antibody (C-Term) at 1:4000 dilutionLane 1: zebrafish muscle lysatesLane 2: Zebrafish lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.