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All lanes : Anti-GIPC1 Antibody (N-term) at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: U-251 MG whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.