Western blot analysis is shown using anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1, 300 dilution of the antibody overnight at 4°C followed by detection using IRDye (TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT. IRDye (TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
