AKT1 non pSer473 (incl. pos. control) [11A11], Mouse Monoclonal Antibody

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Detection of endogenous PKB: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab 11A11 (1 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: Hela; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT; lane 8: Neuro 2A; lane 9: 3T3