Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of CRP in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).