| Purity: | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4�C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80�C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Synonym: | Interleukin-4, Interleukin 4, IL-4, Lymphocyte Stimulatory Factor 1, B_cell Stimulatory Factor 1, B Cell Growth Factor 1, Binetrakin, Pitrakinra, BSF-1, B-Cell Stimulatory Factor 1, Interleukin 4 Variant 2, BCGF-1, BCGF1, BSF1. |
