Applications: | ELISA (recommended work dilution= 1: 8,000), Western Blotting (recommended work dilution= 1:1000-5,000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein ADIPOQ.The membrane blot was probed with anti-ADIPOQ primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant proteinADIPOQ from E.coli cell (15kDa). |