Applications: | ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa). |