All lanes : Anti-XYLT1 Antibody (N-term) at 1:1000 dilutionLane 1: NCI-H460 whole cell lysateLane 2: 293 whole cell lysateLane 3: U-87MG whole cell lysateLane 4: PANC-1 whole cell lysateLane 5: Mouse brain whole cell lysateLane 6: Rat brain whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.