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| Catalogue No |
Partner |
Description |
Species |
Application |
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RB-01-0001-100
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RayBiotech, Inc.
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Mouse anti-Human bFGF/FGF basic/FGF2
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-10-0002-100
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RayBiotech, Inc.
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Rabbit anti-His Tag Antibody
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His-tag Polyclonal Antibody. Recognizes His- tagged recombinant proteins or His-tagged proteins over-expressed in cells.
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Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control, B: transfected
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-10-0002B-50
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RayBiotech, Inc.
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His Tag [+Biotin], Rabbit Polyclonal Antibody
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His-tag Polyclonal Antibody. Recognizes His- tagged recombinant proteins or His-tagged proteins over-expressed in cells.
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Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control, B: transfected
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228-11058-2
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RayBiotech, Inc.
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Lypressin
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228-11251-2
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RayBiotech, Inc.
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Protein Kinase Akt1/PKB alpha, Inactive enzyme, Human Recombinant
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229-20083
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RayBiotech, Inc.
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Purified HAMSTER IgG
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