Applications: | FLISA, IHC-F, IHC-P, ICC |
Dilutions: | FLISA:<br/>ELISA:<br/>FITC conjugate<br/>TRITC conjugate<br/>R-PE conjugate<br/>AP conjugate<br/>HRP conjugate<br/>BIOT conjugate<br/>1:200 – 1:400<br/>1:100 – 1:400<br/>1 ug/mL<br/>1:2,000-1:4,000<br/>1:4,000-1:8,000<br/>1:5,000-1:20,000 |
Immunogen: | Rabbit IgG |
Purification: | Affinity chromatography on rabbit IgG covalently linked to agarose |
Conjugation: | TRITC |
Shipping Conditions: | Ship on cold packs |
Storage: | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C<br/>- The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3<br/>Store at 2- 8 C<br/>- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C<br/>- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C<br/>- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C<br/>- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!<br/> - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
Usage: | Research Use Only |