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RayBiotech

RayBiotech Enzyme Immuno Assay (EIA)

The RayBiotech Enzyme ImmunoAssay (EIA) is an in vitro quantitative assay for detecting Adipokine peptide based on the principle of Competitive Enzyme Immunoassay.  The microplate in the kit is pre-coated with anti-Adipokine polyclonal antibody.  After blocking non-specific binding sites, Adipokine antibody could be competitively bound by both biotinylated adipokine peptide and peptide standard or targeted peptide in samples. The biotinylated Adipokine peptide then interacts with Streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrates. The intensity of chemiluscent signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of adipokine peptide in the standard or samples.  This is due to the competitive binding to Adipokine antibody between biotinylated Adipokine peptide and peptides in standard or samples. A standard curve of known concentration of Adipokine peptide can be established and the concentration of Adipokine peptide in the samples can be calculated accordingly.

How it works
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RayBiotech Adipokine EIAs


Product

Cat#

Format

Peptide MW (Dalton)

Amino Acid

Protein MW (KD)

Sensitivity A

Sensitivity B

Species

Sample Type

Visfatin

EIH-VIS-1

96-well plate

3017.35

491

55.521

379 pg/ml

6.82 pM

mouse, human, rat

cell lysate, supernantant, serum

Ghreilin

EIH-GHR-1

96-well plate

3370.9

117

12.911

161 pg/ml

12.46 pM

mouse, human, rat

cell lysate, supernantant, serum

Nesfatin

EIH-NES-1

96-well plate

1637.84

431

47.5

147 pg/ml

3.09 pM

mouse, human, rat

cell lysate, supernantant, serum

Apelin

EIH-APC-1

96-well plate

1158.33

77

8.569

142 pg/ml

16.57 pM

mouse, human, rat

cell lysate, supernantant, serum

Angiotensin II

EIH-AGII-1

96-well plate

1598.83

485

53.154

2.62 ng/ml

49.29 pM

mouse, human, rat

cell lysate, supernantant, serum

Neuropeptide Y

EIH-NPY-1

96-well plate

1993.19

97

10.851

180 pg/ml

16.59 pM

mouse, human, rat

cell lysate, supernantant, serum

PYY

EIH-PYY-1

96-well plate

4049.55

97

11.046

pg/ml

pM

mouse, human, rat

cell lysate, supernantant, serum

Amylin

EIH-AMY-1

96-well plate

3184.5

89

9.806

pg/ml

pM

mouse, human, rat

cell lysate, supernantant, serum

CART

EIH-CART-1

96-well plate

1158.43

116

12.829

pg/ml

pM

mouse, human, rat

cell lysate, supernantant, serum

Adiponectin/ Acrp30

EIH-ACP-1

96-well plate

1591.78

244

26.414

pg/ml

pM

mouse, human, rat

cell lysate, supernantant, serum

Glucagon-like Peptide 2

EIH-GLP-2

96-well plate

3796.22

180

21.115

pg/ml

pM

mouse, human, rat

cell lysate, supernantant, serum

Reference List
1. Hug C, Lodish HF. Visfatin: a new adipokine. Science. 2005; 307(5708):366-7.
2. Kim MK. Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866. J Mol Biol. 2006; 362(1):66-77.
3. Revollo, J.R., et al. The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells. J. Biol. Chem. 2004; 279: 50754-50763.
4. Oh-I S, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus". Nature 2006; 443 (7112): 709-12.
5. Zhang J, Ren P, Avsian-Kretchmer O, Luo C, Rauch R, Klein C, Hsueh A. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science 2005; 310 (5750): 996-9.
6. Cummings D, Weigle D, Frayo R, Breen P, Ma M, Dellinger E, Purnell J. Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med 2002; 346 (21): 1623-30. 
7. Tschöp M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents. Nature 2002; 407 (6806): 908-913. 
8. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999; 402 (6762): 656-60.

Testing Services
RayBiotech provide a range of EIA testing services.  Please enquire at Insight Biotechnology for more information and pricing.


Benefits of RayBiotech’s EIA Testing Services
  • EIA test provides High specificity and reproducibility
  • Adipokines detected at pg/ml and pmol/L levels
  • Rapid turnover
  • EIA tests are suitable for use with diverse biological samples
  • Low sample volume required
  • Unique targets; no other available assays available

 

Representative Resultshttp://www.raybiotech.com/images/EIA2.gif

 

 

RayBiotech EIA Kits
RayBiotech provide a wide range of simple and easy to use EIA kits (see table above).

Purchase a RayBiotech EIA Kit

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Contents of Kit

  • 96-well Adipokine Microplate (Item A)
  • Wash Buffer Concentrate (20x) (Item B)
  • Standard Adipokine Peptide (Item C)
  • Anti-Nesfatin polyclonal antibody (Item N)
  • Assay Diluent A (Item D)
  • Assay Diluent B (Item E)

 

  • Biotinylated Nesfatin peptide (Item F)
  • HRP-Streptavidin concentrate (Item G)
  • Positive control (Item M)
  • 10.TMB One-Step Substrate Reagent (Item H)
  • 11.Stop Solution (Item I)
  • 13. User Manual (Item K)

 

Please click here for a description of other products and services offered by RayBiotech

 

 


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