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Custom Protein Services:  Baculovirus Expression Systems

Insight Biotechnology provides a broad range of baculovirus protein expression services which can be tailored to your specific research needs.  Choose any starting phase or combination.   Insight Biotechnology takes a flexible approach towards custom protein services and information on this website is only a guide.  Feel free to contact our team of protein expression experts with any further questions.   Email, or telephone Insight Biotechnology on Tel:  +44(0)208 3850303.   

Please Click here for further information about other custom services offered by Insight Biotechnology.

Features of Insight Biotechnology’s Baculoviral Protein Expression Service

Insight Biotechnology’s Custom Baculovirus Protein Expression Service offers the fastest and most cost-effective way for you to take advantage of the Baculovirus expression technology. 

  • Up to 6-fold yield enhancement and fast turnaround time. 
  • Expert technical staff with more than 15 years of experience 
  • Modularised and customised service
  • Competitive price to fit your budget

Available Services are as follows:

Depending on your research needs, you can choose any starting phase or combination.  Typical services:  Baculovirus Expression Full Service Phase II, III and IV for £2550.  Baculovirus Expression Basic Service Phase II for £1175


Phase I: Subcloning into Recombinant Baculovirus Transfer Vector

Description

Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into recombinant Baculovirus transfer vector.

 

Verification of the subcloned gene by restriction digest and sequencing.

 

Preparation of the recombinant vector DNA suitable for co-transfection with baculovirus DNA.

 

 

Required Materials

5µg of plasmid containing gene of interest plus complete vector sequence in electronic format.

 

 

Deliverables

1) 20µg of ready-to-use recombinant Baculovirus transfer plasmid containing the gene of interest

 

2) Final sequencing data

 

3) Detailed research report

 

 

Duration

2 weeks; If insert > 2kb, one week additional per 1kb

 

 

Price

£666; If insert > 2kb, £266 additional per 1kb;


Phase II: Generation and Evaluation of Baculovirus Recombinants

Description

Cotransfection of recombinant Baculovirus transfer vector containing gene of interest with 3 different linearised Baculovirus DNAs. Recombinant clones will be identified and amplified. The customer will choose the best recombinant baculovirus for the protein expression.

 

 

Required Materials

20µg of ready to use recombinant Baculovirus transfer plasmid containing the gene of interest.

Deliverables

1) 3 cell pellets for evaluation of Baculovirus to be used and 50ml recombinant baculovirus solution

 

2) Detailed research report

 

 

Duration

2-3 weeks

 

 

Price

£1175


Phase III: Amplification of Recombinant Baculovirus Stock

Description

Amplification of the chosen recombinant Baculovirus and titration of final high-titre stock by end point dilution.

 

 

Required Materials

At least 5 ml of recombinant Baculovirus stock

 

 

Deliverables

1) Specified quantity (in ml) of high-titre recombinant Baculovirus stock solution ready to express the protein of interest

 

2) Detailed research report

 

 

Duration

2-3 weeks

 

 

Price

£400 for each 500 ml


Phase IV: Amplification of Recombinant Baculovirus Stock

Description

Amplification of the chosen recombinant Baculovirus to a high-titre stock of more than 1 x 108 pfu per ml

 

Infection of three insect cell lines (Sf21, Sf9, and T.ni using the high-titre stock and with or without booster virus

 

Cells are harvested at two different time points (72hr or 96hr)

 

 

Required Materials

At least 5-10 ml of recombinant Baculovirus stock.

 

 

Deliverables

1) 18 sets of cell pellets and media for evaluation of protein yield

 

2) Detailed research report

 

 

Duration

2-3 days

 

 

Price

£1250


Phase V: Protein Production Service

Description

Amplification of recombinant Baculovirus stock for protein production;

 

Expression of recombinant protein under optimised conditions.

 

 

Required Materials

At least 100 ml of high-titre (>108 pfu) recombinant Baculovirus stock. Optimised protein expression conditions are determined by previous evaluation project.

 

 

Deliverables

1) Cell pellets ready for protein purification or in the case of a secreted recombinant protein, the customer will receive the supernatant

 

2) Detailed research report

 

 

Duration

2~3 weeks (1~5 Litres)

 

4~5 weeks (5~20 Litres)

 

8~10 weeks (20 and above Litres)

 

 

Price

£400 for each litre of culture


Phase VI: Protein Extraction & Purification

Description

Cell pellet harvested from 1 litre of insect culture will be lysed with our optimised reagents and protocol.

 

Protein purification using appropriate affinity resin for 6xHis or GST-tagged recombinant proteins.

 

Purification can be performed either under native or denatured conditions (as desired by the customer).

 

SDS Page and Western Blot will be performed for quality testing.

 

 

Required Materials

Cell pellet ready for extraction

 

 

Deliverables

1) Protein solution in standard or customer-specified buffer

 

2) SDS-PAGE or Western Blot data

 

3) Detailed research report

 

 

Duration

2-3 weeks

 

 

Price

£999/L, enquire the price for larger volume

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