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Custom Protein Services: Bacterial Expression Systems

Insight Biotechnology provides a broad range of bacterial protein expression services which can be tailored to your specific research needs.  Choose any starting phase or combination.   Insight Biotechnology takes a flexible approach towards custom protein services and information on this website is only a guide.  Feel free to contact our team of protein expression experts with any further questions.   Email, or telephone Insight Biotechnology on Tel:  +44(0)208 3850303.   

Please Click here for further information about other custom services offered by Insight Biotechnology.

Insight Biotechnology’s Custom Bacterial Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including:

  • Cloning
  • Subcloning
  • Generation of plasmid DNA
  • Multiprotein Complex Expressions
  • Optimisation of expression conditions
  • Small, mini- or large-scale expression and affinity purification
  • Purity evaluation and determination of concentration
  • Lyophilisation of protein, and more.

From Gene to Protein Expression

Construct Generation
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Sequencing Verification of Expression Construct
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Desired Clone Selection and Screening
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Protein Expression and Purification
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Immunological Assay and Evaluation
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Ready for Customer

Outline of Recombinant Protein Expression Service Using Bacterial Systems

Phase Service

Timeline

Description

Price

 

 

Phase 1: Subcloning

 

 

 

1 week

Directly use customer’s clone and expression system without any modification

No charge

Subclone your desired cDNA into an expression vector (Customer provides the plasmid)

 

Enquire

Confirm sequence of the final vector (customer provides the plamid)

Subclone your desired cDNA into an expression vector (plasmid provided)

 

Enquire

Confirm sequence of the final vector

 

 

 

 

 

Phase 2: Small-scale Protein Expression

 

 

1 week

Screen desired clones

 

 

Enquire

Grow clone in 100ml of medium

Express protein in 100ml of medium

Pellet cells, lyse cell pellet, centrifuge and collect supernate

Primary identification of protein expression by immunological assay

 

 

 

 

Phase 3: Large-scale Protein Expression

 

Grow the selected clone in 2L of medium

 

Enquire

Express the protein in 2L of medium

Pellet cells, lyse cell pellet, centrifuge and collect supernate

 

 

 

 

 

Phase 4: Protein purification on a large scale

 

 

1 week

Purify protein from supernates on affinity chromatography column

 

 

Enquire

Identify the fractions containing desired protein using immunological assay, such as Western Blot

Concentrate the pooled fractions

Determine the protein concentration

Yield: Up to 100–500 µg of purified protein

Lyophilise protein to customer’s specifications

Insight Biotechnology also provides a number of additional services, including:

  • Biotinylation of purified  protein
  • Proteolytic cleavage and fragment purification
  • Site directed mutagenesis in customer provided fragment
  • Protein assay development and validation

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