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Custom Antibody Service

Having problems finding a good antibody to your target molecule? Insight Biotechnology has an excellent track record when it comes to generating exceptionally high quality antibodies at the first attempt.

Our fully integrated proteomics service facility utilises modern and innovative technology platforms to meet all your research needs in a cost effective way.  Our consultants will advise on all manner of polyclonal and monoclonal projects, including antibodies raised against proteins and peptides, as well as sites which have been post translationally modified by phosphorylation, methylation, acetylaton and glycosylation. 

We offer a multi-faceted and flexible approach to polyclonal and monoclonal antibody production, tailoring each package towards the specific requirements of your project.

Perhaps your project would be better suited to a non standard host?  In addition to the traditional mouse and rabbit options, Insight Biotechnology also offers rat, chicken and hamster, and is in an excellent position to advise you on the merits and drawbacks of different approaches. 

Contact our scientific team for a free consultation, and to obtain a quote by telephone +44(0)208 385 0303, or email info@insightbio.com.

Package options and prices

Polyclonal packages

Protein to Antisera

Customer provides:

  • Purified and lyophilised protein

Insight Biotechnology Provides:

  • Repeat immunizations of two rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 40 to 60ml of antisera
  • Full project report including ELISA data

Timescale: 11 to 13 weeks

Price:£435

Protein to Antigen Purified Antibody

Customer provides:

  • Purified and lyophilised protein

Insight Biotechnology Provides:

  • Customers protein checked by SDS PAGE
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 3-7mg of Antigen purified antibody
  • Full project report including ELISA data

Timescale: 11 to 13 weeks
Price:£540

Peptide Design to Antisera

Insight Biotechnology Provides:

  • Immunogen design
  • Peptide synthesis and conjugation, up to 22 residues
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 2-3mg peptide
  • 1-2ml of pre-immune sera
  • 40-60ml antisera
  • Full project report including ELISA data

 

Timescale: 12 to 16 weeks
Price:£540

Peptide Design to Antigen Purified Antibody

Insight Biotechnology Provides:

  • Immunogen design
  • Peptide synthesis and conjugation, up to 22 residues
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 2-3mg peptide
  • 1-2ml of pre-immune sera
  • 3-7mg antigen purified antibodies
  • Full project report including ELISA data

Timescale: 13 to 16 weeks
Price:£665

Vector to Antisera

Customer Provides:

  • Vector for bacterial expression of the immunogen protein

Insight Biotechnology Provides:

  • Protein expression and purification of 1mg
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 40 to 60ml of antisera
  • Full project report including ELISA data

Timescale: 13 to 16 weeks
Price:£720

Vector to Antigen Purified Antibody

Customer Provides:

  • Vector for bacterial expression of the immunogen protein

Insight Biotechnology Provides:

  • Protein expression and purification of 1mg
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 3-7mg of Antigen purified antibody
  • Full project report including ELISA data

Timescale: 13 to 16 weeks
Price:£820

cDNA to Antisera

Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)

Insight Biotechnology Provides:

  • Gene amplification and vector construction
  • Protein expression and purification of 1mg
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 40-60ml of antisera
  • Full project report including ELISA data

Timescale: 15 to 17 weeks
Price:£1080

cDNA to Antigen Purified Antibodies

Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)

  • Gene amplification and vector construction
  • Protein expression and purification of 1mg
  • Repeat immunisations of 2 rabbits
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • 1-2ml of pre-immune sera
  • 3-7mg of Antigen purified antibody
  • Full project report including ELISA data

 

Timescale: 15 to 17 weeks
Price:£1180

Phospho Specific Antibodies

Insight Biotechnology Provides:

  • Design of peptide immunogen
  • Peptide synthesis, purification and conjugation
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Antigen affinity purification

Timescale: 15 to 17 weeks
Price:£1160

Western Blot Guaranteed Antibodies

Customer provides:

  • Sample (lysate / tissue homogenate, cell line information etc..)

Insight Biotechnology Provides:

  • Design of peptide immunogen (We would most likely use two immunogen’s in parallel to maximize probability of success)
  • Peptide synthesis, purification and conjugation
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Antigen affinity purification
  • 1-2ml of pre-immune sera
  • 3-7mg of Antigen purified antibody
  • Full project report including ELISA data

Timescale: 15 to 17 weeks
Price:£1360

Western Blot and IHC Guaranteed Antibodies

Customer provides:

  • Sample for Western, in addition to IHC sample

Insight Biotechnology Provides:

  • Design of peptide immunogen (We would most likely use two immunogen’s in parallel to maximize probability of success)
  • Peptide synthesis, purification and conjugation
  • Sera collection
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Antigen affinity purification
  • 1-2ml of pre-immune sera
  • 3-7mg of Antigen purified antibody
  • Full project report including ELISA data

Timescale: 15 to 17 weeks
Price:£1495

Monoclonal packages

Protein to Ascites Production

Customer provides:

  • Purified and lyophilised protein

Insight Biotechnology Provides:

  • Check the customers protein via SDS PAGE
  • Multiple Immunizations
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Fusion and hybridoma development
  • Determination of antibody isotype
  • Purification of two clones and ascites production
  • 50µl of pre immune sera 1:10 dilution
  • 2-5 clones
  • 3-7mg purified antibodies
  • Project report containing ELISA data

 
Timescale: 19 to 25 weeks
Price:£2995

Peptide Design to Ascites Production

Insight Biotechnology Provides:

  • Peptide design, synthesis and conjugation
  • Multiple Immunizations
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Fusion and hybridoma development
  • Determination of antibody isotype
  • Purification of two clones and ascites production
  • 50µl of pre immune sera 1:10 dilution
  • 2-5 clones
  • 3-7mg purified antibodies
  • Project report containing ELISA data

Timescale: 21 to 26 weeks
Price:£3250

Vector to Ascites Production

Customer Provides:

  • Vector for bacterial expression of the immunogen protein

Insight Biotechnology Provides:

  • Protein expression and purification
  • Multiple Immunizations
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Fusion and hybridoma development
  • Determination of antibody isotype
  • Purification of two clones and ascites production
  • 50µl of pre immune sera 1:10 dilution
  • 2-5 clones
  • 3-7mg purified antibodies
  • Project report containing ELISA data

Timescale: 22 to 27 weeks
Price:£3406

cDNA to Ascites Purification

Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)

Insight Biotechnology Provides:

  • Gene amplification
  • Vector construction
  • Protein expression and purification
  • Multiple Immunizations
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Fusion and hybridoma development
  • Determination of antibody isotype
  • Purification of two clones and ascites production
  • 50µl of pre immune sera 1:10 dilution
  • 2-5 clones
  • 3-7mg purified antibodies
  • Project report containing ELISA data

Timescale: 23 to 28 weeks
Price:£3665

Phospho Specific Monoclonal Antibodies

Insight Biotechnology Provides:

  • Peptide design and synthesis (2-3mg of modified and unmodified peptide)
  • Conjugation
  • Multiple Immunizations
  • Continual monitoring of antibody titre via sera collection and ELISA detection
  • Fusion and hybridoma development
  • Determination of antibody isotype
  • Purification of two clones and ascites production
  • 50µl of pre immune sera 1:10 dilution
  • 2-5 clones
  • 3-7mg purified antibodies
  • Project report containing ELISA data

Timescale: 22 to 27 weeks
Price:£3995

 

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Insight Biotechnology has a longstanding relationship with Epitomics, the Rabbit Monoclonal antibody (RaBMab) company, enabling us to provide their revolutionary technology, considerably below the normal market rates.  RaBMabs combine the high affinity of the rabbit immune system with the specificity of a monoclonal antibody, generating phenomenally powerful research and pharmaceutical tools (read more).

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