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Santa Cruz Biotechnology, Inc.

SantaCruz

Santa Cruz Biotechnology is a world leader in the development of products for the biomedical research market. Over the past twenty years, the Company has focused on the ongoing development of research antibodies, biochemicals and labware.

Santa Cruz products are distributed by Insight Biotechnology in the UK and Ireland.    Insight Biotechnology’s Technical Team is happy to discuss how Santa Cruz Biotechnology products can be used to facilitate your research. Contact our scientific team by telephone: +44(0)208 385 0303 or email

Search Santa Cruz products:

A summary of Santa Cruz Biotechnology’s major product ranges is outlined below.

Primary Antibodies

Santa Cruz Biotechnology now offers over 17,708 monoclonal and 38,410 polyclonal antibodies directed to a broad range of mammalian and non-mammalian protein targets. To locate any antibody of interest, enter the product name, catalog number, keyword, or protein name into the search box.  Your results can be further screened using the filters on the side bar.

Secondary Antibodies, Control Antibodies and Normal Sera

Santa Cruz Biotechnology offers a wide variety of secondary antibodies, control immunoglobulin and control sera for a large selection of species, including mouse, rabbit, goat, donkey, chicken, rat, hamster and sheep. Specialized secondary antibodies, such as isotype specific secondary antibodies and F(ab')2 secondary antibodies are available and allow for highly specific results. The Cruz Marker™ secondary antibodies are suitable to use with Cruz Markers™ for viewing molecular weight markers on your exposures. Control immunoglobulin and immunoglobulin conjugates are useful negative controls. Normal sera is offered to be used as blocking reagents.

Phospho-Specific Antibodies

Santa Cruz Biotechnology offers antibodies specific for phosphorylated amino acids tyrosine, serine and threonine in addition to 311 monoclonal antibodies and 1,223 polyclonal antibodies specific for phosphorylated proteins. Antibodies directed to phosphotyrosine, phosphoserine and phosphothreonine are of value in determining whether various proteins become phosphorylated in response to different inducing agents, whereas site specific phospho-specific antibodies provide information regarding activation of target proteins at specific phosphorylation sites. To locate any antibody of interest, enter the product name, catalog number, keyword, or protein name into the search box. Your results can be further screened using the filters on the side bar.

Flow Cytometry and Immunofluorescent Staining

Santa Cruz Biotechnology offers a wide range of primary and secondary antibodies useful for flow cytometry and immunofluorescence microscopy with surface or internal cell staining. Santa Cruz offer an array of primary and secondary antibodies as conjugates with FITC (fluorescein isothiocyanate), TRITC (tetramethylrhodamine isothiocyanate), TR (Texas Red), PE (phycoerythrin), PE-Cy5, PE-Cy7, APC (allophycocyanin), APC-Cy7, AF488 (Alexa Fluor® 488), AF647 (Alexa Fluor® 647), AF405 (Alexa Fluor® 405), PerCP (peridinin chlorophyll protein) and PerCP-Cy5.5.

Antibodies for IHC (Paraffin) Staining

Santa Cruz Biotechnology offers a diverse and wide ranging collection of antibodies useful for immunohistochemical staining of tissues. Paraffin sections can be stained with antibodies specific to a molecular target thereby allowing the microscopic identification of cell and tissue types within the slice, and also the observation of the expression patterns of the target molecule within the cellular and tissue architecture. To locate any antibody of interest, enter the product name, catalog number, keyword, or protein name into the search box. Your results can be further screened using the filters

Post Transcriptional Gene Silencers

Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes. siRNAs are provided as pools of 3 target specific 19-25 nt siRNAs designed to knock down expression of specific gene of interest. Each vial contains 3 nmol of lyophilized siRNA and is sufficient for 50 - 100 transfections. For independent verification of gene silencing results, we also will provide, upon request, individual siRNA duplex components, each containing 3 nmol of lyophilized siRNA. shRNA 0Plasmid DNA Gene Silencers are provided as pools of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNA (sequences correspond to Santa Cruz Biotechnology’s well-established existing product line of siRNA gene silencers). shRNA Plasmids are provided as 20 µg transfection-ready plasmid DNA for either transient or stable (puromycin-selection) target specific gene silencing. shRNA Lentiviral Particles are provided as pools of three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin loop) shRNA and are provided as 200 µl transduction-ready viral particles for gene silencing in mammalian cells (human or mouse). Suitable control antibodies and RT-PCR primers are available for monitoring gene expression knockdown. To locate any post transcriptional silencing product of interest, enter the product name, catalog number, keyword, or the name of the protein target into the search box. Your results can be further screened using the filters on the side bar.

Immunoprecipitation Reagents: protein A, G and L agarose conjugates available

Protein A-Agarose

sc-2001

mouse IgG2a, IgG2b and IgA
rabbit polyclonal Abs
human IgG1,IgG2 and IgG4

100 reactions

Protein G PLUS-Agarose

sc-2002

mouse IgG1, IgG2a,IgG2b and IgG3
rat IgG1, IgG2a,IgG2b and IgG3
rabbit and goat polyclonal Abs
human IgG1, IgG2,IgG3 and IgG4

100 reactions

Protein A/G PLUS-Agarose

sc-2003

all of the above Abs

100 reactions

Protein L-Agarose

sc-2336

mouse, rat, human IgG, scFv and Fab fragments

100 reactions

Tissue Extracts, Cell Lysates and Proteins

Santa Cruz Biotechnology offers a variety of tissue extracts, whole cell lysates, nuclear extracts and proteins for use in combination with their antibodies as Western Blotting or Immunoprecipitation positive controls. Santa Cruz Biotechnology’s nuclear extracts are suitable as either positive controls for Western Blotting or for use in Gel Shift Assays. Biologically active proteins , such as LIF, p53 or p38 manufactured by Santa Cruz Biotechnology, can be used to elicit biological activity or as Western Blotting positive controls. For use as positive controls for phospho-specific antibodies, Santa Cruz Biotechnology offer Phospho-Enriched Whole Cell Lysates and Tissue Extracts.

Transfected Cell Lysates

Santa Cruz Biotechnology, Inc. now offers over 3,375 human and 3,210 mouse transfected lysates for Western blot antibody evaluation. Transfected lysates offer a reliable means to evaluate antibody quality and serve as an excellent positive control for experiments involving antibodies. We believe that our total of over 6,500 well characterized, transfection-designed lysates represent the most extensive offering available to date from any source. All lysates are validated for expression of the correct protein by Western Blot analysis. Appropriate control antibodies for protein expression verification are provided, both on our web site and on lysate product data sheets. Feel free to contact our Technical Department for help locating a suitable transfected lysate.

Specialty Reagents and Buffers

Santa Cruz Biotechnology offers an extensive product line of reagents and buffers for laboratory use, the majority of which have been designed to be used in conjunction with their antibodies and gene silencers to achieve optimal results. Santa Cruz Biotechnology’s Specialty Reagent product line includes Immunoprecipitations Reagents (such as Protein A/G PLUS Agarose), ABC and ImmunoCruz™ Staining Systems for staining paraffin-embedded tissues, standard buffers (such as PBS, TBS and RIPA Lysis buffer), support reagents for our gene silencers, molecular weight standards and biologically active proteins and peptides.

For additional technical information and to view products and prices please enter the product name, catalog number, keyword, or protein name into the search box. Your results can be further screened using the filters on the side bar. Can’t find the right product on our website? Contact our scientific team by telephone: +44(0)208 385 0303 or email info@insightbio.com.

 


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