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Allele

Presenting: An immobilized GFP-binding protein derived from camelid single domain antibodies
Optimized by the research team at Allele Biotechnology, GFP-nAb™ is a highly specific GFP (Green Fluorescent Protein) binding protein derived from camelids. It is characterized by a small size (13 KDa) and a very high stability (stable up to 70°C, functional in high salt concentrations or 0.5% SDS). One molecule of GFP-nAb™ binds one molecule of GFP with a dissociation constant (Kd) in the sub nanomolar range. This makes GFP-nAb™ resin the ideal candidate for a variety of biological assays, some of which are listed below:

  • Immunoprecipitation / CO-IP
  • Quantitative analysis
  • Chromatin Immunoprecipitation (ChIP)
  • Identifying Interacting Proteins
  • RIP Assays (RNA Immunoprecipitation)
  • CLIP Assays (in vivo Cross Linking and Immunoprecipitation)

GFP-nAb™ binds a large number of commonly used fluorescent proteins derived from the original Aequorea victoria GFP, including EGFP, Venus, Cerulean, EBFP2 and more. Conveniently, it displays no binding affinity to non-jellyfish-derived fluorescent proteins, including the “mFruits” such as mCherry, or Allele’s mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The GFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions, allowing you to perform multiple immunoprecipitation reactions in a matter of minutes.

Request your Complementary Sample Vial of GFP-nAb Today

Insight Bio has 100 complementary sample size vials of GFP-nAb available to customers on a first come, first serve basis. Contact our PhD level technical team today by telephone +44(0)208 385 0303 or email info@insightbio.com for further details

  Allele Complete Pulldown using the GFP-nAb™ Spin Kit.
EGFP-expressing Sf9 (insect) cell lysate contained a total
of 16µg of EGFP in total volume of 500µl, determined
spectrophotometrically. Following the GFP-nAb™ Spin Kit
binding and wash protocols, the protein was eluted in 2
x 50 µl elution buffer (0.2M glycine pH 2.5), pooled, and
neutralized with 10µl of 1M Tris base. Equal volumes of lysate
input (I), flow-through (FT) after binding to GFP-nAb™
agarose resin, and elution (E) fractions were analyzed by
SDS-PAGE followed by Coomassie staining. In this experiment,
EGFP was quantitatively removed from the lysate.
 

Product Code

Description

Size

Price

ABP-NAB-GFPAK20

GFP-nAb™ Agarose Spin Kit (20 Reactions)

20 Rxns

£342

ABP-NAB-GFPA025

GFP-nAb™ Agarose 0.25ml

250μl

£160

ABP-NAB-GFPA050

GFP-nAb™ Agarose 0.5ml

500μl

£291

ABP-NAB-GFPA100

GFP-nAb™ Agarose 1ml

1ml

£524

ABP-NAB-GFPAB

ABP-NAB-GFPAB

250μg

£145

ABP-NAB-GFPM025

GFP-nAb™ Magnetic 0.25ml

250μL

£160

 

 


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