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GenScript

GenEZ™ ORF Clone Cloning Vectors

GenEZ™ ORF clones offer the highest flexibility in ORF clone customization. Choose from our standard mammalian expression-ready cloning vector or from over 100 popular commercial cloning vectors for custom cloning.

Standard expression cloning vector

  • Expression/transfection ready cloning vector for mammalian systems

GenScript's GenEZ™ ORF clones are seamlessly cloned into our standard mammalian expression cloning vector, pcDNA3.1+/C-(K)-DYK, using our CloneEZ™ seamless cloning technology. Driven by a CMV promoter, pcDNA3.1+/C-(K)-DYK is equipped with an C-terminal DYKDDDDK tag for easy protein detection and purification. GenEZ™ ORF clones are delivered expression and transfection ready, saving researchers valuable time in the lab. There is no additional charge for cloning into pcDNA3.1+/C-(K)-DYK.

Expression System
Promoter
Bacterial Selection
Copy number
Epitope tag
Length
Mammalian
CMV
Ampicillin
High
C-DYK
5444

Custom cloning vectors

  • Competitively priced subcloning
  • Choose from 180 popular commercial vectors, download vector list (.xls)
  • Add up to 30 bp of 5' and 3' flanking sequence for free

For enhanced flexibility, GenEZ™ ORF clones can be directly custom cloned into one of over 180 expression vectors that we offer compatible with mammalian or bacterial systems including pcDNA, pET, pPIC, and pUC vectors series.

In addition, for GenEZ™ ORFs cloned into custom vectors can be further customized by the addition of up to 30 bp of 5' or 3' flanking sequence – free of charge.

Selected Custom Cloning Vectors

Vector name Expression System Promoter Bacterial Selection Copy number Epitope tag Length
pCDNA3.1+-N-DYK
Mammalian
CMV
Ampicillin
High
N-DYK
5438
pcDNA3.1+N-HA
Mammalian
CMV
Ampicillin
High
N-HA
5450
pcDNA3.1+N-His
Mammalian
CMV
Ampicillin
High
N-6*HIS
5441
pcDNA3.1+N-Myc
Mammalian
CMV
Ampicillin
High
N-MYC
5453
pcDNA3.1+N-GST(TEV)
Mammalian
CMV
Ampicillin
High
N-GST-(TEV)
6092
pcDNA3.1+N-GST(Thrombin)
Mammalian
CMV
Ampicillin
High
N-GST-(Thrombin)
6080
pcDNA3.1+C-HA
Mammalian
CMV
Ampicillin
High
C-HA
5441
pcDNA3.1+C-His
Mammalian
CMV
Ampicillin
High
C-6*HIS
5432
pcDNA3.1+C-Myc
Mammalian
CMV
Ampicillin
High
C-MYC
5444


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