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GenScript

GenEZ™ ORF cDNA Mutant Clones

GenEZ™ Mutant ORF clones allow you to create any custom mutant you desire, while eliminating the use of expensive, time-consuming, unpredictable mutagenesis kits and protocols. Create custom point mutations, insertions, and deletions, from any wild type ORF sequence found in our ORF clone database.

Unlike off-the-shelf cDNA clones from other companies, GenScript's ORF clones are totally customizable., allowing you to design mutant ORFs wild type sequences and add up to 30 bp of flanking sequence to the 5' and 3' ends of the ORF. Choose from over 180 popular commercial vectors including mammalian and bacterial expression vectors, or standard cloning vectors. GenEZ™ ORF cDNA clones start at just $99/clone and 5-day turnaround time, with delivery of 10μg sequence-verified plasmid DNA.

GenEZ™ Mutant ORF Clones

  • Mutations include: point mutations, insertions, deletions, truncations
  • Expression/transfection ready
  • Quote and order online in ~ 5 minutes

Deliverables

  • 10 μg of lyophilized plasmid DNA
  • Sequence chromatograms covering your gene (electronic)
  • Construct map for the plasmid (electronic)
  • Quality assurance certificate
Mutation type*
Description and Applications
Mutation Definition
Point mutations
Change single base pairs to study key amino acids.
Any number of point mutations within a 30 bp region
Insertions
Insert bases to add signal peptides, create frameshifts, or simulate repeat expansions,
Any insertion of up to 30 bp
Truncations / Deletions
Create constructs to express protein domains. Remove fragments from the N-terminal, C-terminal, or middle of your gene. Delete single bases to create frameshifts or remove single amino acids.
Removal of a single fragment, regardless of length

*1 mutation equals:

  • any combination of base changes within a 30-base frame
  • removal of a single continuous fragment regardless of length

GenEZ™ Mutant ORF clone advantages

  • Expression/transfection ready: Choose from > 100 popular commercial vectors
  • 100% sequence-verified ORFs
  • No need for expensive, unpredictable mutagenesis kits and protocols

GenEZ™ Mutant ORF Clone Deliverables*

  1. 10 µg research grade wild type ORF lyophilized plasmid (gene sequence in vector of choice)
  2. 4 µg research grade mutant ORF lyophilized plasmid (gene sequence in vector of choice)
  3. Sequencing chromatograms (electronic)
  4. Construct maps (electronic)
  5. Quality Assurance Certificate


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