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Global 5mC ELISA
DNA Methylation

MethylFlash™ -- A Tried & True Technique!

Not all DNA methylation studies require long and cumbersome techniques like NGS. Sure, you'll get an abundance of data (to tediously dig through), but it comes with a "nifty" price tag. Many times, all that's needed is a simple, practical method for a general overview -- take our global DNA methylation and hydroxymethylation ELISAs. These MethylFlash(TM) kits have been a trusted technique for quantifying 5-mC and 5-hmC in DNA samples for years, with results comparable to that of HPLC [1][2]. Plus, they won't cost you a "pretty penny" and can get you the results you need in a "jiffy".

"Here's a tip: A global DNA methylation assay is a fast and cheap pre-screening method prior to tedious and costly next-gen sequencing"


Measure global 5mC, 5hmC, or 5fC in less than 4 hours

» View Side-by-Side Comparison

Fig. 1. Comparison of MethylFlash technology to HPLC. Peer-reviewed publication showed that the MethylFlash quantification kit in Jenkins' [1] study indicated a 0.04% level of 5-hmC in leukocytes as compared to a similar, independent study by Guz [2] which indicated a 0.03% level using HPLC, demonstrating that the MethylFlash kit is a comparable method to HPLC.
1. Jenkins TG et al: Fertil Steril. 2013 Oct;100(4):945-51
2. Guz J et al: Biol Reprod. 2014 Jul 24


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