Custom Antibody Production
Having problems finding a good antibody to your target molecule? Insight Biotechnology has an excellent track record when it comes to generating exceptionally high quality antibodies at the first attempt.
Our fully integrated proteomics service facility utilizes modern and innovative technology platforms to meet all your research needs in a cost effective way. Our consultants will advise on all manner of polyclonal and monoclonal projects, including antibodies raised against proteins and peptides, as well as sites which have been post translationally modified by phosphorylation, methylation, acetylaton and glycosylation.
We offer a multi-faceted and flexible approach to polyclonal and monoclonal antibody production, tailoring each package towards the specific requirements of your project.
Perhaps your project would be better suited to a non standard host? In addition to the traditional mouse and rabbit options, Insight Biotechnology also offers rat, chicken and hamster, and is in an excellent position to advise you on the merits and drawbacks of different approaches.
Contact our scientific team for a free consultation, and to obtain a quote by telephone +44 20 8385 0303, or email info@insightbio.com.
Package Options:
Polyclonal packages
Customer provides:
- Purified and lyophilised protein
Insight Biotechnology Provides:
- Repeat immunizations of two rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 40 to 60ml of antisera
- Full project report including ELISA data
Timescale: 11 to 13 weeks
Please contact us for a quoteCustomer provides:
- Purified and lyophilised protein
Insight Biotechnology Provides:
- Customers protein checked by SDS PAGE
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 3-7mg of Antigen purified antibody
- Full project report including ELISA data
Timescale: 11 to 13 weeks
Please contact us for a quote
Insight Biotechnology Provides:
- Immunogen design
- Peptide synthesis and conjugation, up to 22 residues
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 2-3mg peptide
- 1-2ml of pre-immune sera
- 40-60ml antisera
- Full project report including ELISA data
Timescale: 12 to 16 weeks
Please contact us for a quote
Insight Biotechnology Provides:
- Immunogen design
- Peptide synthesis and conjugation, up to 22 residues
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 2-3mg peptide
- 1-2ml of pre-immune sera
- 3-7mg antigen purified antibodies
- Full project report including ELISA data
Timescale: 13 to 16 weeks
Please contact us for a quote
Customer Provides:
- Vector for bacterial expression of the immunogen protein
Insight Biotechnology Provides:
- Protein expression and purification of 1mg
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 40 to 60ml of antisera
- Full project report including ELISA data
Timescale: 13 to 16 weeks
Please contact us for a quote
Customer Provides:
- Vector for bacterial expression of the immunogen protein
Insight Biotechnology Provides:
- Protein expression and purification of 1mg
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 3-7mg of Antigen purified antibody
- Full project report including ELISA data
Timescale: 13 to 16 weeks
Please contact us for a quote
Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)
Insight Biotechnology Provides:
- Gene amplification and vector construction
- Protein expression and purification of 1mg
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 40-60ml of antisera
- Full project report including ELISA data
Timescale: 15 to 17 weeks
Please contact us for a quote
Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)
- Gene amplification and vector construction
- Protein expression and purification of 1mg
- Repeat immunisations of 2 rabbits
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- 1-2ml of pre-immune sera
- 3-7mg of Antigen purified antibody
- Full project report including ELISA data
Timescale: 15 to 17 weeks
Please contact us for a quote
Insight Biotechnology Provides:
- Design of peptide immunogen
- Peptide synthesis, purification and conjugation
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Antigen affinity purification
Timescale: 15 to 17 weeks
Please contact us for a quote
Customer provides:
- Sample (lysate / tissue homogenate, cell line information etc..)
Insight Biotechnology Provides:
- Design of peptide immunogen (We would most likely use two immunogen’s in parallel to maximize probability of success)
- Peptide synthesis, purification and conjugation
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Antigen affinity purification
- 1-2ml of pre-immune sera
- 3-7mg of Antigen purified antibody
- Full project report including ELISA data
Timescale: 15 to 17 weeks
Please contact us for a quote
Customer provides:
- Sample for Western, in addition to IHC sample
Insight Biotechnology Provides:
- Design of peptide immunogen (We would most likely use two immunogen’s in parallel to maximize probability of success)
- Peptide synthesis, purification and conjugation
- Sera collection
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Antigen affinity purification
- 1-2ml of pre-immune sera
- 3-7mg of Antigen purified antibody
- Full project report including ELISA data
Timescale: 15 to 17 weeks
Please contact us for a quote
Monoclonal packages
Customer provides:
- Purified and lyophilised protein
Insight Biotechnology Provides:
- Check the customers protein via SDS PAGE
- Multiple Immunizations
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Fusion and hybridoma development
- Determination of antibody isotype
- Purification of two clones and ascites production
- 50µl of pre immune sera 1:10 dilution
- 2-5 clones
- 3-7mg purified antibodies
- Project report containing ELISA data
Timescale: 19 to 25 weeks
Please contact us for a quote
Insight Biotechnology Provides:
- Peptide design, synthesis and conjugation
- Multiple Immunizations
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Fusion and hybridoma development
- Determination of antibody isotype
- Purification of two clones and ascites production
- 50µl of pre immune sera 1:10 dilution
- 2-5 clones
- 3-7mg purified antibodies
- Project report containing ELISA data
Timescale: 21 to 26 weeks
Please contact us for a quote
Customer Provides:
- Vector for bacterial expression of the immunogen protein
Insight Biotechnology Provides:
- Protein expression and purification
- Multiple Immunizations
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Fusion and hybridoma development
- Determination of antibody isotype
- Purification of two clones and ascites production
- 50µl of pre immune sera 1:10 dilution
- 2-5 clones
- 3-7mg purified antibodies
- Project report containing ELISA data
Timescale: 22 to 27 weeks
Please contact us for a quote
Customer Provides:
cDNA (it is possible we can either purchase this or obtain it for free, please enquire)
Insight Biotechnology Provides:
- Gene amplification
- Vector construction
- Protein expression and purification
- Multiple Immunizations
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Fusion and hybridoma development
- Determination of antibody isotype
- Purification of two clones and ascites production
- 50µl of pre immune sera 1:10 dilution
- 2-5 clones
- 3-7mg purified antibodies
- Project report containing ELISA data
Timescale: 23 to 28 weeks
Please contact us for a quote
Insight Biotechnology Provides:
- Peptide design and synthesis (2-3mg of modified and unmodified peptide)
- Conjugation
- Multiple Immunizations
- Continual monitoring of antibody titre via sera collection and ELISA detection
- Fusion and hybridoma development
- Determination of antibody isotype
- Purification of two clones and ascites production
- 50µl of pre immune sera 1:10 dilution
- 2-5 clones
- 3-7mg purified antibodies
- Project report containing ELISA data
Timescale: 22 to 27 weeks
Please contact us for a quote
Contact Us
Tel: 0800 073 3133 (UK)
Fax: 0800 953 0268 (UK)
Tel: +44 20 8385 0303 (International)
Email: Info@insightbio.com
