Insight Biotechnology : : limited supply high quality reagents for cell and molecular biology, specializing in antibodies for medical research.

Absolute S specific instructions, flow
Flow cytometry assay protocol for measuring cell proliferation without any heat or acid-denaturation steps. Surface and internal epitopes are preserved.

Antigen surface staining, flow cytometry

ApoBrdU specific instructions, flow
A two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.

ApoDirect specific instructions, flow
A two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.

Apoptosis induction, using EOS9.1
CD95 (Fas)-induced apoptosis of human Jurkat cell line by monoclonal antibody EOS9.1 is used as a general positive control for apoptosis detection systems.

Baculovirus Protein Expression & Analysis

Bioassays, cytokine
Measurement of biological activity of cytokines and their concentrations by cellular proliferation of primary cell cultures or isolated cell lines that are dependent and/or responsive to a specific growth factor.

Common Fluorescent Dyes in Flow Cytometry

Cross-Reactivity of Anti-Human/Mouse Antibodies

Cytokine ELISA, antibody pairs
Used for the detection and quantification of cytokines secreted into tissue culture supernatant and/or biological fluids. Using two antibodies specific for non-overlapping epitopes allows the optimal capture and detection of the desired cytokine.

Cytokine intracellular staining, flow cytometry
A modification of the basic immunofluorescence staining and flow cytometric analysis used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level.

Cytokine neutralizing protocols
Protocols describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies.

Cytokine Ready-SET-Go! Protocol

ELISPOT (Enzyme-linked ImmunoSPOT)
Used to obtain colorimetric footprint of B cells that can be quantitated by visual scoring or specialized plate-readers.

Immunoblotting Protocol (Western Blot)

Immunohistochemistry (IHC) Staining, Frozen Tissues

Immunoprecipitation Protocol
This technique provides a rapid and simple means to separate a specific protein from whole cell lysates or culture supernatants. Additionally, immunoprecipitation can be used to confirm the identity or study biochemical characteristics, post-translational modifications, and expression levels of a protein of interest.

In Vivo Cytokine Capture Assay (IVCCA)

In vivo use of antibodies
Antibodies are used for in vivo studies where the injected antibody is intended to either deplete a specific cell type or induce activation of cells. eBioscience offers monoclonal antibodies to mouse cell surface antigens and cytokines for in vitro or in vivo functional studies.

T Cell Activation using anti-CD3 mAb
Protocol A: Stimulation of mouse peripheral T cells with plate-bound 145-2C11 monoclonal antibody; MTT assay for detection of cellular proliferation.
Protocol B: Stimulation of human peripheral blood mononuclear cells with anti-human CD3 monoclonal antibody; MTT assay for detection of cellular proliferation.

RBC Lysis

Thawing & Freezing Protocols for insect cells (BV)

Toll-Like Receptor (TLR) Detection, flow cytometry

Toll-Like Receptor 2 (TLR2) Neutralization Bioassay

Toll-Like Receptor 4 (TLR4) Neutralization Bioassay
TLR4 antibodies are used to inhibit the LPS-induced cytokine production by human PBMCs.

Epitomics (RabMabs) Protocols

Immunocytochemistry (Immunofluorescence)

Immunohistochemical Staining of Paraffin Embedded Tissues

Immunoprecipitation

Flow Cytometry - Intracellular Staining

Western Blotting

Frequently Asked Questions (FAQs)