Glass Chip-Based Cytokine Antibody Arrays

RayBiotech’s G series antibody arrays utilise a glass slide platform, which delivers easy handling as well as speed and consistency for microarray-based assays.  It is particularly suitable for projects with very limited amounts of samples (as little as 10µl per assay) and a large number of samples (several dozens of assays can be processed per day by a single technician without any difficulty).  Furthermore, since it consumes much less reagents, the price is very comparable with our popular membrane based antibody arrays (averaging approximately 20% less than membrane-based arrays).

The principle of G series antibody arrays (glass slide-based antibody arrays) is similar to membrane based antibody arrays.  The whole experimental procedure is simple and can be performed at any laboratory with a simple device (G series accessories).  After blocking arrays with blocking buffer, the arrays are incubated with samples.  Unspecific bound proteins are then removed and the arrays are incubated with a cocktail of biotin-antibodies and then cy3 streptavidin.  Signals are visualised using a laser scanner.  Although G series antibody arrays require a laser scanner for signal detection, if you do not have a laser scanner, RayBiotech also provides scan service.  Please enquire at Insight Biotechnology.

Please click here for further information and pricing on RayBiotech’s Cytokine Antibody Array Testing Service.

RayBiotech provide a wide range of simple and easy to use Glass Slide Based Antibody Array Kits.

Please click here for a description of other products and services offered by RayBiotech.

RayBiotech G-Series Antibody Array Technology

Selected References Citing RayBiotech’s Cytokine Antibody Arrays

Kocaoemer A, Kern S, Klüter H, Bieback K  (2007)  Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue.  Stem Cells:25 5 1270-8

Huang RP  (2007)  An array of possibilities in cancer research using cytokine antibody arrays.  Expert Rev Proteomics:2 299-308
Huang R, Lin Y, Shi Q, Flowers L, Ramachandran S, Horowitz IR, Parthasarathy S, Huang RP  (2004)  Enhanced protein profiling arrays with ELISA-based amplification for high-throughput molecular changes of tumor patients' plasma.  Clin Cancer Res:10 2 598-609

Agrawal N, Bettegowda C, Cheong I, Geschwind JF, Drake CG, Hipkiss EL, Tatsumi M, Dang LH, Diaz LA Jr, Pomper M, Abusedera M, Wahl RL, Kinzler KW, Zhou S, Huso DL, Vogelstein B (2004)  Bacteriolytic therapy can generate a potent immune response against experimental tumors.  Proc Natl Acad Sci U S A:101 42 15172-7

Carroll TP, Greene CM, Taggart CC, Bowie AG, O'Neill SJ, McElvaney NG  (2005)  Viral inhibition of IL-1- and neutrophil elastase-induced inflammatory responses in bronchial epithelial cells.  J Immunol:175 11 7594-601

De S, Razorenova O, McCabe NP, O'Toole T, Qin J, Byzova TV  (2005)  VEGF-integrin interplay controls tumor growth and vascularization.  Proc Natl Acad Sci U S A:102 21 7589-94

Kim BG, Li C, Qiao W, Mamura M, Kasprzak B, Anver M, Wolfraim L, Hong S, Mushinski E, Potter M, Kim SJ, Fu XY, Deng C, Letterio JJ  (2006)  Smad4 signalling in T cells is required for suppression of gastrointestinal cancer.  Nature:441 1015-1019

Marks DJ, Harbord MW, MacAllister R, Rahman FZ, Young J, Al-Lazikani B, Lees W, Novelli M, Bloom S, Segal AW  (2008)  Defective acute inflammation in Crohn's disease: a clinical investigation.Marks DJ  (2008)  Lancet:367 9511 668-78

Tang X, Marciano DL, Leeman SE, Amar S  (2005) LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines.  Proc Natl Acad Sci U S A:102 14 5132-7

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