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RayBiotech Membrane Based Cytokine Antibody Arrays RayBiotech’s membrane based cytokine antibody array technology is based on the sandwich immunoassay principle. A panel of antibodies (capture) is immobilised in specific spot locations on the surface of a membrane. Membranes are incubated with biological samples, resulting in capture of cytokines by corresponding antibodies. The bound cytokines are subsequently detected with a cocktail of biotinylated antibodies. Signals are then visualised using chemiluminescence or colourimetry or infrared fluorescence. RayBiotech’s standard products apply chemiluminescence detection. If you prefer colourimetric or infrared detection this can be arranged, please contact Insight Biotechnology.
RayBiotech provide a wide range of Membrane based Cytokine Antibody Array Kits. Please click here for more information and pricing on RayBiotech’s Cytokine Antibody Array Testing Service. Please click here for a description of other products and services offered by RayBiotech. Membrane Based Cytokine Antibody Array Technology
Membrane Array Results Specificity and sensitivity are two major concerns for designing protein arrays using antibodies as capture and detection reagents. RayBiotech only select antibodies with the highest specificity and affinity in their array system. All antibodies used in RayBiotech’s system are rigidly tested and must meet their superior standards. Examples below show the highest standard of array systems. High Specificity A recombinant protein IL-8 was incubated with an array membrane spotted with 82 capture antibodies. The signal was only detected in the IL-8 antibody spot. All antibodies in the system were tested in this way. High Sensitivity Cytokines are detected with very high sensitivity using the array format. Cytokine antibody array membranes were incubated with different concentrations of MCP-1. The intensities of signal were measured by densitometry and plotted against the concentration of MCP-1. High Reproducibility High reproducibility is important in experimentation. The human Cytokine Antibody Array System 1 was probed with conditioned medium. Three membranes showed similar signal intensities. Comparable to ELISA data The relative expression levels in protein arrays were determined by densitometry. The actual amounts of protein levels were quantified by ELISA. Fold change represents cytokines differentially expressed between TNF-alpha treated and untreated conditioned media (U251 cells) and between patient sera (number 4 and number 11).
This technology has proven reliability with over 200 publications in journals like Nature, Nature Medicine, PNAS, JBC and Lancet
Examples of Membrane Arrays
Partial List of Papers Citing RayBiotech Products Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, Boxer A, Blennow K, Friedman LF, Galasko DR, Jutel M, Karydas A, Kaye JA, Leszek J, Miller BL, Minthon L, Quinn JF, Rabinovici GD, Robinson WH, Sabbagh MN, So YT, Sparks DL, Tabaton M, Tinklenberg J, Yesavage JA, Tibshirani R, Wyss-Coray T Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. (2007) Nat Med:11 1359-62 Agrawal N, Bettegowda C, Cheong I, Geschwind JF, Drake CG, Hipkiss EL, Tatsumi M, Dang LH, Diaz LA Jr, Pomper M, Abusedera M, Wahl RL, Kinzler KW, Zhou S, Huso DL, Vogelstein B (2004) Bacteriolytic therapy can generate a potent immune response against experimental tumors. Proc Natl Acad Sci U S A:101 42 15172-7 Carroll TP, Greene CM, Taggart CC, Bowie AG, O'Neill SJ, McElvaney NG (2005) Viral inhibition of IL-1- and neutrophil elastase-induced inflammatory responses in bronchial epithelial cells. J Immunol:175 11 7594-601 De S, Razorenova O, McCabe NP, O'Toole T, Qin J, Byzova TV (2005) VEGF-integrin interplay controls tumor growth and vascularisation. Proc Natl Acad Sci U S A:102 21 7589-94 Kim BG, Li C, Qiao W, Mamura M, Kasprzak B, Anver M, Wolfraim L, Hong S, Mushinski E, Potter M, Kim SJ, Fu XY, Deng C, Letterio JJ (2006) Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Nature:444 7120-780 Marks DJ, Harbord MW, MacAllister R, Rahman FZ, Young J, Al-Lazikani B, Lees W, Novelli M, Bloom S, Segal AW (2008) Defective acute inflammation in Crohn's disease: a clinical investigation.Marks DJ (2008) Lancet:367 9511 668-78 Tang X, Marciano DL, Leeman SE, Amar S (2005) LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines. Proc Natl Acad Sci U S A:102 14 5132-7 Wang FX, Xu Y, Sullivan J, Souder E, Argyris EG, Acheampong EA, Fisher J, Sierra M, Thomson MM, Najera R, Frank I, Kulkosky J, Pomerantz RJ, Nunnari G (2005) IL-7 is a potent and proviral strain-specific inducer of latent HIV-1 cellular reservoirs of infected individuals on virally suppressive HAART. J Clin Invest:115 1 128-37 Xu Y, Kulkosky J, Acheampong E, Nunnari G, Sullivan J, Pomerantz RJ (2004) HIV-1-mediated apoptosis of neuronal cells: Proximal molecular mechanisms of HIV-1-induced encephalopathy. Proc Natl Acad Sci U S A:101 18 7070-5 Huang RP (2007) An array of possibilities in cancer research using cytokine antibody arrays. Expert Rev Proteomics 4 2 299-308 Please click here for a description of other products and services offered by RayBiotech.
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