RayBiotech Enzyme Immuno Assay (EIA)

The RayBiotech Enzyme ImmunoAssay (EIA) is an in vitro quantitative assay for detecting Adipokine peptide based on the principle of Competitive Enzyme Immunoassay.  The microplate in the kit is pre-coated with anti-Adipokine polyclonal antibody.  After blocking non-specific binding sites, Adipokine antibody could be competitively bound by both biotinylated adipokine peptide and peptide standard or targeted peptide in samples. The biotinylated Adipokine peptide then interacts with Streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrates. The intensity of chemiluscent signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of adipokine peptide in the standard or samples.  This is due to the competitive binding to Adipokine antibody between biotinylated Adipokine peptide and peptides in standard or samples. A standard curve of known concentration of Adipokine peptide can be established and the concentration of Adipokine peptide in the samples can be calculated accordingly.

How it works

RayBiotech Adipokine EIAs

Reference List
1. Hug C, Lodish HF. Visfatin: a new adipokine. Science. 2005; 307(5708):366-7.
2. Kim MK. Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866. J Mol Biol. 2006; 362(1):66-77.
3. Revollo, J.R., et al. The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells. J. Biol. Chem. 2004; 279: 50754-50763.
4. Oh-I S, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus". Nature 2006; 443 (7112): 709-12.
5. Zhang J, Ren P, Avsian-Kretchmer O, Luo C, Rauch R, Klein C, Hsueh A. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science 2005; 310 (5750): 996-9.
6. Cummings D, Weigle D, Frayo R, Breen P, Ma M, Dellinger E, Purnell J. Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med 2002; 346 (21): 1623-30. 
7. Tschöp M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents. Nature 2002; 407 (6806): 908-913. 
8. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999; 402 (6762): 656-60.

Testing Services
RayBiotech provide a range of EIA testing services.  Please enquire at Insight Biotechnology for more information and pricing.

Representative Results

RayBiotech EIA Kits
RayBiotech provide a wide range of simple and easy to use EIA kits (see table above).

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