Vantage™ microRNA Labelling Kit

Marligen’s Vantage™ microRNA Labelling Kit utilizes a proprietary labelling technology to provide exceptional sensitivity for the detection of endogenous microRNAs.  This method can label any RNA sample including total RNA, enriched low molecular weight (LMW) RNA, amplified sense RNA and degraded RNA.  The simple protocol consists of just two steps.  The 3’end of the RNA is poly(A) tailed followed by the ligation of biotinylated 3DNA™ dendrimers containing 15 biotins to provide signal amplification.

Advantages:

• Labelling can be completed in 45 minutes
• Highly sensitive labelling method requires little input RNA (as little as 100g total RNA extracted from tissues)
• LMW RNA enrichment not necessary
• Labels degraded RNA from FFPE samples
• Biotinylated RNA suitable for multiple applications
• Compatible for use with xMAP® microRNA profiling kits

Labelling completed in 45 minutes

This simple protocol consists of just two steps. The 3’end of the RNA is poly(A) tailed followed by the ligation of biotinylated 3DNA™ dendrimers containing 15 biotins to provide signal amplification.

Highly sensitive labelling method requires little input RNA

Marligen’s Vantage™ microRNA labelling and detection kits can detect <1pg (or <250 attomoles) synthetic microRNA.  In this example, the limit of detection of miR-210 is 0.09pg (12 attomoles) of synthetic miR.

Labels degraded RNA

Total RNA was extracted from normal and tumour lung.  The total RNA from both samples was determined to be degraded when analyzed using Agilent LabChips®. The RNA samples were labelled and detected using Marligen’s Vantage™ microRNA Labelling Kit and Oncology Detection Panel 1 (Cat. No. 11831-050). A subset of microRNAs detected by the Vantage™ Oncology Panel  1 is shown here.  The data demonstrates that expression profiles from degraded RNA can be analyzed using the Marligen Vantage™ labelling and detection method.

Order Vantage™ microRNA Labelling Kit

This product or portions thereof is manufactured under license from Genisphere, Inc. Marligen Biosciences, Inc. is the exclusive licensee for the research use of this labelling technology in microRNA Luminex™ bead-based assays. 3DNA™ and Genisphere® are trademarks of Datascope.

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