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 Marligen

Marligen’s Vantage Total RNA Purification Kit provides a rapid method for the isolation and purification of high quality total RNA from a variety of different sample types without the use of phenol or chloroform.

Product features:

(1) Spin-column format allows processing of multiple samples in less than 20 minutes

Total RNA Process

(2) Isolates all RNA species including large (e.g. mRNA) and LMW RNAs (e.g. miRNAs, siRNAs)

Purified total RNA analyzed on an agilent RNA labchip®®

 (3) Excellent yields from a variety of sample types

Comparison of marligen and qiagen kits]

Total RNA Yields from the Vantage™ Total RNA Purification Kit

Type

Quality

Yield RNA

Cells

 

 

Hepatocytes

1 x 106 cells

30µg

NIH/3T3 Cellws

5 x 105 cells

4.4µg (nanodrop)

Serum or Plasma

100µl

200-300ng

Stem Cells

50,000 cells

2µg

HeLa

1 x 106 cells

15µg

MCF-7 cells

1 x 106 cells

10 µg

E. Coli

1 x 109 cells

50µg

S. cerevisiae

1 x 107 cells

30 µg

Tissues

 

 

Brain

10mg

8 µg

Kidney

10mg

30 µg

Liver

10mg

30 µg

Lung

10mg

10µg

Spleen

10mg

35µg

(4) Comparison of RT-PCR products generated from total RNA purified using Marligen’s kit, a leading competitor's kits and the Trizol extraction method.

4 RT-PCR experiment with range of RNA sizes 

(5) Total RNA can be isolated from small amounts of sample – even from a single cell.
Total RNA purified from a single cell can be analyzed by qRT-PCR

(6) Total RNA can be purified from a diverse array of sample types including cultured cells, tissues, blood, bacteria, yeast, fungi, plants and nasal or throat swabs.

Total RNA isolated from a variety of different plant tissues

Total RNA isolated from yeast

Please click here to purchase Marligen’s RNA purification products

Please click here for a description of other products and services offered by Marligen.

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