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Custom Protein Services: Bacterial Expression Systems
Insight Biotechnology provides a broad range of bacterial protein expression services which can be tailored to your specific research needs. Choose any starting phase or combination. Insight Biotechnology takes a flexible approach towards custom protein services and information on this website is only a guide. Feel free to contact our team of protein expression experts with any further questions. Email, or telephone Insight Biotechnology on Tel: +44(0)208 3850303.
Please Click here for further information about other custom services offered by Insight Biotechnology.
Insight Biotechnology’s Custom Bacterial Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including:
- Cloning
- Subcloning
- Generation of plasmid DNA
- Multiprotein Complex Expressions
- Optimisation of expression conditions
- Small, mini- or large-scale expression and affinity purification
- Purity evaluation and determination of concentration
- Lyophilisation of protein, and more.
From Gene to Protein Expression
Construct Generation |
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Sequencing Verification of Expression Construct |
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Desired Clone Selection and Screening |
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Protein Expression and Purification |
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Immunological Assay and Evaluation |
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Ready for Customer
Outline of Recombinant Protein Expression Service Using Bacterial Systems |
Phase Service |
Timeline |
Description |
Price |
Phase 1: Subcloning |
1 week |
Directly use customer’s clone and expression system without any modification |
No charge |
Subclone your desired cDNA into an expression vector (Customer provides the plasmid) |
£333.34 |
Confirm sequence of the final vector (customer provides the plamid) |
Subclone your desired cDNA into an expression vector (plasmid provided) |
Enquire |
Confirm sequence of the final vector |
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Phase 2: Small-scale Protein Expression |
1 week |
Screen desired clones |
£133.34 |
Grow clone in 100ml of medium |
Express protein in 100ml of medium |
Pellet cells, lyse cell pellet, centrifuge and collect supernate |
Primary identification of protein expression by immunological assay |
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Phase 3: Large-scale Protein Expression |
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Grow the selected clone in 2L of medium |
£266.67 |
Express the protein in 2L of medium |
Pellet cells, lyse cell pellet, centrifuge and collect supernate |
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Phase 4: Protein purification on a large scale |
1 week |
Purify protein from supernates on affinity chromatography column |
£800.00 |
Identify the fractions containing desired protein using immunological assay, such as Western Blot |
Concentrate the pooled fractions |
Determine the protein concentration |
Yield: Up to 100–500 µg of purified protein |
Lyophilise protein to customer’s specifications |
Insight Biotechnology also provides a number of additional services, including:
- Biotinylation of purified protein
- Proteolytic cleavage and fragment purification
- Site directed mutagenesis in customer provided fragment
- Protein assay development and validation
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