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Custom Antibody Service
Having problems finding a good antibody to your target molecule? We can generate almost any kind of antibody, including recombinant, engineered and Rabbit Monoclonal.
Insight Biotechnology has an excellent track record when it comes to generating exceptionally high quality antibodies at the first attempt. We provide a range of polyclonal, monoclonal and recombinant antibody production services. Our consultants are happy to advise customers on all manner of projects, including antibodies raised against proteins and peptides, as well as sites which have been post translationally modified by phosphorylation, methylation, acetylaton and glycosylation. Insight Biotechnology also has a great deal of experience when it comes to generating bioactive and idiotype antibodies.
Perhaps your project would be better suited to a non standard host? In addition to the traditional mouse and rabbit options, Insight Biotechnology also offers rat, chicken and hamster, and is in an excellent position to advise you on the merits and drawbacks of different approaches. Likewise, our highly experienced immunogen design consultants are always happy to give you the benefit of their considered advice.
Insight Biotechnology offers a range of antibody purification methods including Protein A/G and antigen purification, as well as antigen synthesis and antibody conjugation. Our consultants are aware that a flexible approach is required in order to meet the varied needs of each custom antibody project. Our antibody production team is happy to discuss your project and tailor a competitively priced package, which best meets the technical needs of you project.
Additionally, Insight Biotechnology is pleased to provide our customers with access to revolutionary Rabbit Monoclonal Antibody technology from Epitomics, read more...
For a consultation on your custom project, or to obtain a quote, please enquire at info@insightbio.com or telephone 0208 3850303.
Please click here to download a custom antibody order form.
Outline of Antibody Generation Service |
Service Items |
Time |
Description |
Part 1:
Polyclonal Antibody |
5-10 weeks |
Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titre, affinity purity |
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Part 2A:
Mouse Monoclonal Antibody |
4-5 months |
Recognizing one antigenic binding site of the antigen, Immunization, fusion, screening, subclone, characterization and affinity purity |
Part 2B:
Rabbit Monoclonal Antibody |
6 months |
Several levels of service are offered and this service can be customised to meet your specific needs. |
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Part 3: Recombinant
Antibody |
6 weeks |
Recognizing one or two antigenic binding site of the antigen, Peptide Design and synthesis, selecting, screening, characterization, affinity purity, sequence and analysis of antibody gene |
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Part 4: Phage Display |
6-8 weeks |
Creating antibody binding to any chosen target antigen without the use of laboratory animals or hybridomas |
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Part 5:
Antibody Engineering |
variable |
Isolation of genes encoding the specific antibody, creating the ability to redesign, enhance or alter specific antibody function based on antibody gene |
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Part 6:
Antibody Purification and Conjugation |
2-3 days |
Conjugating polyclonal, monoclonal and recombinant antibodies, with Enzymes, fluorochromes, affinity ligand and solid surface conjugation |
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Part 1: Polyclonal Antibody (Comprehensive Price List) |
Service Items |
Time |
Criteria |
Price |
Anti-regular peptide antibody |
5 weeks |
- 4 mg of peptide from customer
- Immunization of two rabbits
- 100ml of immune sera
- Generated antibody recognizes immunogen in ELISA assay
- ELISA titre is up to 1:10000
- Antibody purification by affinity column may be obtained upon request
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£400 |
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Anti-regular peptide antibody |
8 weeks |
- Design, synthesize and conjugate peptide(15-20mer,5-10mg)
- Immunisation of two rabbits
- 100ml of immune sera
- Generated antibody recognises immunogen in ELISA assay
- ELISA titre is up to 1:10000
- Antibody purification by affinity column may be obtained upon request
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£800 |
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Anti-phospho-peptide antibody |
8-10 weeks |
- Design, synthesize, specific site phophorylation and conjugate peptide(12-15mer,5-10mg)
- Immunisation of two rabbits
- 100ml of immune sera
- Generated antibody recognises immunogen in ELISA assay
- ELISA titre is up to 1:10000
- Antibody purification by affinity column may be obtained upon request
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£1533.33 |
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Anti-Protein Antibody |
5-7 weeks |
- 1.5mg of purified protein from customer
- Immunisation of two rabbits
- 100ml of immune sera
- Generated antibody recognises immunogen in ELISA assay.
- ELISA titre is up to 1:8000
- Antibody purification by affinity column may be obtained upon request
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£400 |
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Part 2A: Mouse Monoclonal Antibody (Comprehensive Price List) |
Service Items |
Time |
Criteria |
Price |
Anti-regular peptide antibody (customer provide antigen) |
4-5 months |
- Customer provide peptide(4mg)
- Immunization of two mice, fusion, screening, subclone and initial characterization
- With up to three monoclones and their supernatant for confirming detection
- Generated antibody recognizes immunogen in ELISA assay
- Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
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£2600 |
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Anti-regular peptide antibody (We provide antigen) |
4-5 months |
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£3066.66 |
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Anti-phospho-peptide antibody |
5-6 months |
- Design, synthesize, specific site phosphorylation and conjugate peptide(11-15mer, 5 mg)
- Immunization of two mice, fusion, screening, subclone and initial characterization
- With up to three monoclones and their supernatant for confirming detection
- Generated antibody recognizes immunogen in ELISA assay
- Antibody purification by affinity column(1-5mg) may be obtained upon request
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£3733.33 |
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Anti-protein antibody |
5 months |
- 4mg of purified Protein from customer Immunization of two mice, fusion, screening, subclone and initial characterization
- Immunization of two mice, fusion, screening, subclone and initial characterization
- With up to three monoclones and their supernatant for confirming detection
- Generated antibody recognizes immunogen in ELISA assay
- Antibody purification by affinity column(1-5mg) may be obtained upon request
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£2660 |
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Part 2B: Rabbit Monoclonal Antibody (For lowest price possible, contract these services through Insight Biotechnology)
Insight Biotechnology is pleased to provide our customers with access to revolutionary Rabbit Monoclonal Antibody technology from Epitomics. This proprietary technology is considered, by many leading scientists to be the future of monoclonal antibody production. Using this platform, it is possible to generate antibodies which have higher affinities and lower backgrounds on a routine basis, when compared with those produced using standard techniques such as mouse hybridoma technology.
Using Rabbit Monoclonal Technology, customers can contract development of custom antibodies for diverse applications right from the conceptual stage of the project. These range from simple immuno-precipitation and Western blotting, to more complex applications such as immuno-fluorescence and immuno-histochemistry, as well as bioactive and idiotype antibodies. With this technology is also possible to generate high quality antibodies raised against sites which have been post translationally modified by phosphorylation, methylation, acetylation and glycosylation. Epitomics can also generate these types of antibodies with the previously stated applications in mind, right from the outset.
To get an idea of the quality of Epitomics existing Rabbit Monoclonal Antibody range just take a look at their catalogues. Download Epitomics IHC catalogue. Download Epitomics Winter Catalogue. Download Epitomics Summer Catalogue |
Stage |
Description |
Customer provides protein, peptide or antigen |
- Immunization of rabbits
- Isolation of lymphocytes
- Conduct fusion
- Conduct ELISA screen
- Send supernatant of 20 positive hybridoma binders for each antigen to Collaborator for additional assays.
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At this point the customer conducts additional assays and selects 3 hybridomas |
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- Sub-clone the hybridomas. Screen sub-clones, by ELISA and select 3 sub-clones from each hybridoma.
Further assays / analysis of subclones |
At this point the customer conducts additional assays and selects 1 sub-clone for each antigen. Additional sub-clones may be purchased |
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- Send the 1 selected hybridoma to Collaborator or produce MAbs
upon request (additional fee). |
The whole process takes 6 month (4 months for delivery of hybridoma supernatant(s) |
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Part 3: Recombinant Antibody |
Why Use Recombinant Antibody:
- Generating antibody without using animal
- Unstable hybridoma lines or primary fusion cultures can be saved by rescuing and cloning the antibody gene
- The size and specificity of Recombinant antibody are smaller and higher than traditional antibody (polyclonal or monoclonal), so that it may allow for attachment or penetration to cell or target sites
- Monoclonal isotype switching and affinity optimization using recombinant DNA techniques
- The purified antibody gene can be mutated to effect binding specificity or neutralizing capabilities
- Increase assay sensitivity through antibody manipulation.
- Create multiple binding antibody
- Create antibody-fusion protein or peptide
- Unlimited supply of antibody through bacterial culture
- Removes the expense and issues associated with ascites production in mice or serum from animals
- Allow to generate antibody to all targets, even small molecules
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Service Items |
Time |
Criteria |
Price |
Anti-regular peptide antibody |
6 weeks |
- Design, synthesize and biotinylated peptide(15-20mer,5-10mg)
- Selecting, screening, and initial characterization
- With up to five ELISA positive clones
- 10 ml of Phage and soluble antibody for customer confirming detection
- Antibody gene sequence and analysis for specific clone
- Antibody purification by affinity column may be obtained upon request
- Customer clone ownership agreement
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Please Enquire |
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Anti-protein antibody |
6 weeks |
- 5mg of purified protein from customer and biotinylated it
- Selecting, screening, and initial characterization
- With up to five ELISA positive clones
- 10 ml of Phage and soluble antibody for customer confirming detection
- Antibody gene sequence and analysis for specific clone
- Antibody purification by affinity column may be obtained upon request
- Customer clone ownership agreement
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Please
Enquire |
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Single chain antibody from hybridoma |
2-3 months
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- Establish specific immunoassay based on customer’s requirement (fixing amount of antigen)
- Grow hybridoma in-house and evaluate antibody secretion ability
- Purify mRNA and synthesize cDNA
- Amplify specific antibody gene by PCR, assemble and cloning them into expressing vector
- Selecting, screening, and initial characterization of ScFv format antibody derived from parent hybridoma
- With up to five ELISA positive clones
- 10 ml of Phage and soluble antibody for customer confirming detection
- Antibody gene sequence and analysis of specific characterized clone
- Antibody purification by affinity column may be obtained upon request
- Customer clone ownership agreement
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Please Enquire |
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Single chain antibody from immunized mouse |
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- 1. 5mg of antigen from customer
- Immunise two mice
- Collect immune sera and establish specific immunoassay
- Collect spleen and blood B cell, purify mRNA and synthesize cDNA
- Amplify specific antibody gene by PCR,
- Construct specific-antigen antibody library
- Selecting, screening, and initial characterization of ScFv format antibody from the displayed library
- With up to five ELISA positive clones
- 10 ml of Phage and soluble antibody for customer detection
- Antibody gene sequence and analysis for specific clone
- Antibody purification by affinity column
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Please
Enquire |
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Single chain antibody based on available gene |
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- 1 mg of antigen and 10 µg of purified DNA from customer
- Design specific primers and amplify specific antibody gene by PCR
- Assemble and cloning them into expressing vector
- Selecting, screening, and initial characterization of ScFv format antibody
- With ELISA positive clones
- 10 ml of Phage and soluble antibody for customer detection
- Antibody gene sequence and analysis for specific clone
- Antibody purification by affinity column
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Please Enquire |
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Single chain antibody based on synthetic DNA |
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- Design and synthesize oligonucleotides based on customer requirement
- Establish specific immunoassay with antigen from customer
- Ligate oligonucleotides, amplify DNA by PCR, assemble and cloning them into expressing vector
- Selecting, screening, and initial characterization of ScFv format antibody from the expressing clones
- With ELISA positive clones
- Verify cloning antibody gene by sequence
- 10 ml of Phage and soluble antibody for customer detection
- Antibody gene sequence and analysis for specific clone
- Antibody purification by affinity column
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Please Enquire |
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Antibody Humanisation |
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- Antigen, antibody, parent clones with murine antibody gene, clear sequence and suggesting immunoassay from customer
- Confirm antibody binding activity with establishing in-house specific immunoassay
- Sequence comprehensive analysis by our tools
- Humanization working strategy and plan
- Design and synthesize a set of primers
- Specific sites mutation, antibody activity and sequence confirm after each cycle mutation.
- First hand of humanization modified plan
- Selecting, screening, and extensive characterization of humanized ScFv format antibody from the mutated library
- Desired clones further evaluation
- Antibody purification by affinity column
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Please Enquire |
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Part 4. Antibody Phage Display
Phage display provides a direct physical link between phenotype and genotype by utilizing the ability to display genes on the surface of bacteriophage. Why use phage display to make recombinant antibody? Traditional ways for preparing antibodies are cumbersome, labour intensive, amount limited and unstable of antibody and animal use. It is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-passes the immune system. Therefore, antibodies can be displayed on phage in form of single chain antibody (scFv) or Fab' fragments using the system. It allows us to quickly construct different format of antibody libraries. It gives new possibilities for providing novel binders to almost any antigen, including those that are poorly immunogenic or non-immunogenic, self-antigen or 'difficult' targets.
Selection and screening of phage-displayed antibody
We provide all antibody displayed services as follows:
1. Further modification of well-characterized antibody or antigen by phage display
2. Creation of target-based mutated antibody phage display library
3. Quick selection and screening of Single chain antibody from antibody library
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Part 5. Antibody Engineering
In antibody engineering, molecular biology approaches are used to improve the function of antibody molecules by altering their amino acid sequence or fussing them to other functional peptides or proteins. The affinity and specificity with which antibodies recognize antigens, their stability in various environmental conditions, their therapeutic efficacy, and their detection in diagnostic applications may thus be enhanced. The platform provides opportunity for the isolation of genes encoding the specific antibody, which therefore creates the ability to redesign, enhance or alter specific antibody function.
The Following are suitable for creating modified antibodies:
- Well characterized non-human antibody
- Unique monoclonal antibody produced from unstable hybridoma
- Want to change your antibody format
- Improve antibody functions
- Convert scFv to whole IgG
We also offer customized antibody engineering including techniques of antibody sequence cloning, converting monoclonal antibody into single chain antibody, humanization and generation of antibody constructs, such as creation of multivalent, multi-specific or multifunctional antibody derivatives or fusion-antibody. Please enquire at Insight Biotechnology for details.
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Part 6. Antibody Purification and Conjugation
All formats of antibodies can be purified by affinity column before labelling. We provide conjugating polyclonal, monoclonal and recombinant antibodies, with the following labels:
- Enzymes, including alkaline phosphatase and horseradish peroxidase, etc.
- Fluorochromes: FITC and Cyanine dyes (Cy3, Cy5 and Cy7 ), etc.
- Affinity ligand conjugation: Biotin, streptavidin, protein A, etc.
- Solid surface: Quantum Dot, Agarose beads, magnetic beads, etc
- Others available upon request
Our antibody conjugates are used for a number of applications, including flow cytometry, ELISA, Western blotting, immunohistochemistry and membrane assays.
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Please click here for a description of other custom products and services offered by Insight Biotechnology.
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