Marligen’s cDNA Construction Services

Marligen will construct cDNA libraries for you from the tissues, cells, or RNA that you provide. They can also purify total RNA or mRNA from your tissues as well as offer cDNA library amplification.

Read more about Marligen’s cDNA Library Construction Services

Libraries are produced using an oligo dT primer-adapter and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA from mRNA.  After the second strand is synthesized, the double stranded cDNA is size fractionated, directionally cloned into our Express 1 vector, and transformed into T1 phage resistant E.coli. Click for pExpress-1 vector DNA sequence or Map and Data.

Please inquire at technical support at info@insightbio.com to for information about special vector or host cell requirements.

Ordering Information

Please contact Insight Biotech on +44(0)20 8385 0303 or e-mail our customer service representatives (info@insightbio.com ) for further information on Marligen’s cDNA Library Construction services and for quote requests.

All cDNA library construction orders are subject to the Marligen Biosciences cDNA Library Construction Provision of Services Agreement.

To initiate your order please complete Marligen Biosciences Custom cDNA Libraries Order Form and fax to 301-874-4993 or e-mail the completed form to info@insightbio.com.  Our customer service representative will contact you to verify your order.

To ensure that we understand the goals of your project and when to expect your samples, please DO NOT send materials until we contact you.

Turn Around

Turnaround time for standard and large-insert libraries is 6-8 weeks and 8-10 weeks for normalized or subtracted libraries. Your custom cDNA Library will be provided to you with a Certificate of Analysis.

Please click here for a description of other products and services offered by Marligen.