Transcription Factor Microplate Assays

Marligen’s Chemiluminescent Transcription Factor Microplate Assays allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells and are designed to be used in combination with Marligen’s Nuclear Extraction Kit to ensure optimal performance.
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Advantages:

• Up to 100-fold more sensitive than gelshift assays
• Eliminates radioactivity and the use of gels
• Superior sensitivity minimizes amount of nuclear extracts required
• Results in less than four hours
• Compatible with standard plate luminometers
• Assays with multiple species including humans, monkeys and rodents
• Direct quantitation of binding activity without the need for antibodies.

Currently, Marligen offers 11 different assays in multiplex format. These assays include AP-1, CRE-ATF, CREB, EGR, GATA, MEF2, Myc-Max, NF-1, NFκB, Octamer, and PPAR. Click here to obtain more information on the sites used for these assays.

Click here for Transcription Factor Microplate Assays

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